TABLE 1.
Purity, integrity, and number of sequencing reads for RNA obtained from nucleated cells and cytoplasts harboring or not L. amazonensis after 48 h of in vitro culturea
| Sample | Organism(s) | A260/A280 ratio | RIN | No. of reads (in millions) | Notes |
|---|---|---|---|---|---|
| L929 fibroblasts | M. musculus | 2.21 | 10 | 101 | |
| M. musculus | 2.10 | 9.1 | 129 | ||
| M. musculus | 2.09 | 9.7 | 194 | ||
| L929 fibroblasts hosting L. amazonensis | M. musculus, L. amazonensis | 2.12 | 9.4 | 275 | Around 40% cells hosting parasites |
| M. musculus, L. amazonensis | 2.09 | 9.3 | 292 | Around 40% cells hosting parasites | |
| M. musculus, L. amazonensis | 2.12 | 9.2 | 305 | Around 40% cells hosting parasites | |
| L929 cytoplasts | M. musculus | 2.05 | 7.8 | 174 | >90% of enucleated cells |
| M. musculus | 2.39 | 7.8 | 182 | >90% of enucleated cells | |
| L929 cytoplasts hosting L. amazonensis | M. musculus, L. amazonensis | 2.72 | 7.6 | 317 | >90% of enucleated cells, 50% cytoplasts hosting parasites |
| M. musculus, L. amazonensis | 2.19 | 7.9 | 232 | Around 90% of enucleated cells, 70% cytoplasts hosting parasites | |
| M. musculus, L. amazonensis | 2.48 | 7.6 | 212 | Around 90% of enucleated cells, 70% cytoplasts hosting parasites |
a
Evaluations were performed on an Agilent Bioanalyzer 2100 instrument. Notes indicate the efficiency of enucleation (the percentage of cytoplasts among the population of enucleated fibroblast cultures) and the percentage of infected cells/cytoplasts.