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. 2020 Oct 5;11:570380. doi: 10.3389/fimmu.2020.570380

Figure 1.

Figure 1

The effects of NK cells and NK cells pre-incubated with DHA on neutrophil expression of CD11b (A) and CD47 (B). Neutrophils were culture alone (NP) or with NK cells that had been pre-incubated for 18 h without (C-NK+NP) or with 50 µM of docosahexaenoic acid (DHA-NK+NP) and then stimulated with IL-2 (2 ng/ml), IL-12 (2 ng/ml), and IL-15 (10 ng/ml). The cells were cultured together for 0, 3, 6, 12, 18, and 24 h. Expression levels were determined by flow cytometry and are presented as mean fluorescence intensity (MFI). Representative overlay histograms of CD11b and CD47 expression levels at 12 h (C). Representative dot plots of expression levels of CD11b and CD47 at 12 h (D). Positive gating was determined with appropriate isotype controls. Data are shown as mean ± SEM; * indicates difference between C-NK+NP and NP, $ difference between DHA-NK+NP and NP, and # difference between DHA-NK+NP and C-NK+NP. One symbol indicates p < 0.05, two symbols p < 0.01, and three symbols p < 0.001. n = 6 (independent donors), collected in three independent experiments.