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. 2020 Oct 5;11:570380. doi: 10.3389/fimmu.2020.570380

Figure 2.

Figure 2

The effects of NK cells and NK cells pre-incubated with DHA on neutrophil production of IL-8 (A), IL-1ra (B), and CXCL10 (C). Neutrophils (NP) and NK cells (C-NK) were cultured alone or together after the NK cells had been pre-incubated for 18 h without (C-NK+NP) or with 50 µM docosahexaenoic acid (DHA-NK+NP) and then stimulated with IL-2 (2 ng/ml), IL-12 (2 ng/ml), and IL-15 (10 ng/ml). The cells were cultured together for 18 h. Cytokine concentrations in supernatants were determined by ELISA and are presented as pg/ml. Data are shown as mean ± SEM; * indicates difference between C-NK+NP and NP, and $ difference between DHA-NK+NP and NP. One symbol indicates p < 0.05 and three symbols p < 0.001. One outlier was removed from the NP group in A and B and one outlier in the C-NK group in C. n = 5 - 11 (except for C-NK in A, n = 3), collected in 4 independent experiments. In Supplementary Figure 2 all outliers are included.