Skip to main content
. 2020 Oct 5;11:570380. doi: 10.3389/fimmu.2020.570380

Figure 3.

Figure 3

The effects of NK cells and NK cells pre-incubated with DHA on neutrophil apoptosis. Neutrophils were cultured alone (NP), or with NK cells that had been pre-incubated for 18 h without (C-NK+NP) or with 50 µM docosahexaenoic acid (DHA-NK+NP) and then stimulated with IL-2 (2 ng/ml), IL-12 (2 ng/ml), and IL-15 (10 ng/ml). The cells were cultured together for 0, 3, 6, 12, 18, and 24 h. Apoptosis was determined by flow cytometric analysis of annexin V (Anx V) binding to neutrophils and are presented as Anx V+ neutrophils (A). Representative dot plots of Anx V binding to neutrophils after 6 and 18 h of co-culture (B). Positive gating was determined with an unstained control. Data are shown as mean ± SEM; * indicates difference between C-NK+NP and NP, $ difference between DHA-NK+NP and NP, and # difference between DHA-NK+NP and C-NK+NP. One symbol indicates p < 0.05, two symbols p < 0.01, and three symbols p < 0.001. n = 8 (independent donors), collected in 4 independent experiments.