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. 2020 Oct 5;11:570380. doi: 10.3389/fimmu.2020.570380

Figure 4.

Figure 4

The effects of NK cells and NK cells pre-incubated with DHA on neutrophil phagocytosis (A) and reactive oxygen species production (B). Neutrophils were cultured alone (NP) or with NK cells that had been pre-incubated for 18 h without (C-NK+NP) or with 50 µM docosahexaenoic acid (DHA-NK+NP) and then stimulated with IL-2 (2 ng/ml), IL-12 (2 ng/ml), and IL-15 (10 ng/ml). The cells were cultured together for 6 h. Phagocytosis was determined by flow cytometric analysis of engulfed FITC-labelled E. coli and presented as percent of the total granulocyte population (A). Reactive oxygen species production was measured by DCFDA conversion to the highly fluorescent 2′,7′-dichlorofluorescein, determined by flow cytometry and presented as percent of the total neutrophil population (B). Positive gating was determined by appropriate untreated controls. Data are shown as mean ± SEM; * indicates difference between C-NK+NP and NP and $ difference between DHA-NK+NP and NP. One symbol indicates p < 0.05. n = 8 (independent donors), collected in three independent experiments.