FIGURE 9.

Atherosclerotic plaque calcification in T cell recipient mice. Representative photographs of aortic sinuses stained with the calcium specific Alizarin Red S in adjuvant (A, inset 20× magnification) or mCRAMP (B) T cell recipient mice. Bar = 0.1 mm. Intracellular staining for splenic CD8+INF-γ+ T cells (C) and CD8+IL-10+ T cells (D). Intracellular staining for splenic CD4+INF-γ+ T cells (E) and CD4+IL-10+ T cells (F). Adjuvant N = 8; mCRAMP N = 14. *P < 0.05, Mann-Whitney test. Gating scheme for T cell intracellular staining shown in Supplementary Figure 8.