Table 2.
Sequences of primer pairs used for amplification of target and reference genes.1
| Gene2 | Strand | Sequence (5´ → 3´) | Ta | Product size (bp) | GenBank Accession No |
|---|---|---|---|---|---|
| ANXA1 | Forward | CTGCCTGACTGCCCTTGTGA | 63 | 98 | NM_206906.1 |
| Reverse | GTTTGTGTCGTGTTCCACTCCC | ||||
| TRAF3 | Forward | CTGAGAAAAGATTTGCCAGACCA | 63 | 101 | XM_421378 |
| Reverse | CATGAAACCATGACACACGGG | ||||
| MUC2 | Forward | ATGCGATGTTAACACAGGACTC | 60 | 110 | BX930545 |
| Reverse | GTGGAGCACAGCAGACTTTG | ||||
| CLDN1 | Forward | CATACTCCTGGGTCTGGTTGGT | 60 | 100 | NM_001013611.2 |
| Reverse | GACAGCCATCCGCATCTTCT | ||||
| OCLDN | Forward | CGCAGTCCAGCGGTTACTA | 58 | 178 | NM_205128.1 |
| Reverse | AGGATGACGATGAGGAACCCA | ||||
| GAPDH | Forward | TTGTCTCCTGTGACTTCAATGGTG | 63 | 128 | NM_204305 |
| Reverse | ACGGTTGCTGTATCCAAACTCAT | ||||
| β-Actin | Forward | CCTGGCACCTAGCACAATGAA | 63 | 175 | NM_205518.1 |
| Reverse | GGTTTAGAAGCATTTGCGGTG |
1For each gene the primer sequence for forward and reverse (5´ → 3´), the product size (bp), and the annealing temperature (Ta) in °C are shown.
2ANXA1, annexin A1; TRAF3, tumor necrosis factor receptor associated factor 3; MUC2, mucin2; CLDN1, claudin1; OCLDN, occludin; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase.