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. 2020 Oct 19;10:17618. doi: 10.1038/s41598-020-74634-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression and cytokine production levels in artificial psoriatic human skin. (A) Relative mRNA expression levels among the artificial psoriatic skin specimens with and without treatment. (i–vi) mRNA expression levels of caspase-14, filaggrin, involucrin, psoriasin, koebnerisin 15L and koebnerisin 15S, respectively. (B) Pro- and anti-inflammatory cytokines in the supernatants of the artificial psoriatic skins with or without treatment for 8 days. (i–xiii) Results of the Bio-Plex Multiplex immunoassay for tumour necrotic factor (TNF)-α, interferon (IFN)-γ, interleukin (IL)-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-17, IL-21 and IL-22.