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. 2020 Oct 20;38:101766. doi: 10.1016/j.redox.2020.101766

Fig. 4.

Fig. 4

LOC344887 knockdown increases the expression of CDH2/N-Cadherin. (A) A heat map showing the expression of CDH1 and CDH2 in A549 LOC344887-WT and LOC344887-KO cells. (B–D) HFL1 cells transfected with LOC-siRNA-#5/#8 (10 nM) for 72 h were harvested for qRT-PCR (B) and immunoblot analysis (C, quantification in D). Herein, AML12, a liver epithelial cell line, was used as a positive control for E-Cad detection. (E–F) HFL1 cells transfected with CDH2-siRNA-#5/#6/#10/#11 (10 nM) for 72 h were harvested for immunoblot analysis (E, quantification in F). (G–H) HFL1 cells co-transfected with LOC-siRNA-#5 and CDH2-siRNA-#5 (10 nM) for 72 h were harvested for immunoblot analysis (G, quantification in H).