Supplementary Figure 3.

Vil1 promoter-driven Cre recombinase expression in the bladder, kidney, and female reproductive organs varies between transgenic lines. (A–C) Cre-dependent GFP reporter expression was detected throughout the uterine epithelium in some Vil1^Cre/20 R26^mTmG/+ mice (such as example shown here), but not the other 2 lines examined. (D–F) Cre-dependent TdTomato reporter expression (pseudo-colored green) is seen in patchy areas of the oviduct epithelium in Vil1^Cre/1000 R26^Ai9 mice, but not the other 2 lines. (G–I) Cre-dependent GFP reporter expression was detected throughout the bladder epithelium in Vil1^Cre/20 R26^mTmG/+ mice, but not the other 2 lines. (J–L) Cre-dependent reporter expression was seen in scattered cells in the kidneys of Vil1^Cre/1000 R26^Ai9 mice and was extensive throughout the renal proximal tubular epithelium in Vil1^Cre/20 R26^mTmG/+ mice. No reporter expression was detected in Vil1^CreERT2/23 R26^mTmG/+ mouse kidney. Scale bars = 25 μm.