Supplementary Figure 4.

The Vil1 promoter drives Cre recombinase expression in the male, but not female, gonads in some transgenic lines. (A, D, G) Cross sections of tissue from adult Vil1^Cre/1000 R26^Ai9/+ mice show no evident Cre-dependent TdTomato reporter expression (pseudo-colored green) in the testis or the ovary. (B, E, H) Cross sections of tissue from adult Vil1^Cre/20 R26^mTmG/+ mice show Cre-dependent GFP reporter expression (green) in subset of seminiferous tubules of the testis but no expression in the ovary. (C, F, I) Cross sections of tissue from adult Vil1^CreERT2/23 R26^mTmG/+ mice 7 days after tamoxifen show Cre-dependent GFP reporter expression (green) in subset of seminiferous tubules of the testis but none in the ovary. Boxed regions in A–C are shown at higher magnification in D–F. Scale bars = 25 μm.