Table 1.

Age-dependent increase of mtDNA heteroplasmy incidence in lymphoblast samples

	Model 1		Model 2		Model 3
mtDNA heteroplasmy	Beta [95% CI]	P	Beta [95% CI]	P	Beta [95% CI]	P
VAF ≥ 1%	0.012 [0.005–0.020]	0.00063	0.012 [0.005–0.019]	0.00073	0.014 [0.005–0.022]	0.0012
VAF ≥ 2%	0.017 [0.007–0.027]	0.00087	0.017 [0.007–0.026]	0.00091	0.020 [0.009–0.031]	0.00043
VAF ≥ 5%	0.027 [0.013–0.042]	0.00025	0.027 [0.013–0.042]	0.00026	0.037 [0.020–0.054]	2.7 × 10−5

The associations between heteroplasmy incidence and age were computed by using Poisson log-linear model with adjustment for sex and sequencing coverage in model 1, and for sex, sequencing coverage, and the relative mtDNA content (STAMP-CN) in model 2 and model 3. In model 3, only heteroplasmies that occurred once in the 182 lymphoblast samples were used to compute the incidence.