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. 2020 Sep 30;20(6):308. doi: 10.3892/ol.2020.12171

Table I.

Primers utilized for reverse transcription-quantitative PCR amplification.

Gene Upstream primer sequence (5′-3′) Downstream primer sequence (5′-3′)
MCM2 ATCTACGCCAAGGAGAGGGT GTAATGGGGATGCTGCCTGT
MCM3 TGGCCTCCATTGATGCTACC GGACGACTTTGGGACGAACT
MCM5 TCATCTCCAAGAGCATCGCC CCTCGGCGAGTAAGTCCATC
CHK1 AATGCTCGCTGGAGAATTGC CACCACCTGAAGTGACTCGG
DHX9 GCCAATTTCTGGCCAAAGCA CGAGGCTCAATGGGGAGTTT
β-actin CAGGCACCAGGGCGTGAT TAGCAACGTACATGGCTGGG

MCM, minichromosome maintenance complex component; CHK1, checkpoint kinase 1; DHX9, DEAH (Asp-Glu-Ala-His) box helicase 9.