Table I.
Gene | Upstream primer sequence (5′-3′) | Downstream primer sequence (5′-3′) |
---|---|---|
MCM2 | ATCTACGCCAAGGAGAGGGT | GTAATGGGGATGCTGCCTGT |
MCM3 | TGGCCTCCATTGATGCTACC | GGACGACTTTGGGACGAACT |
MCM5 | TCATCTCCAAGAGCATCGCC | CCTCGGCGAGTAAGTCCATC |
CHK1 | AATGCTCGCTGGAGAATTGC | CACCACCTGAAGTGACTCGG |
DHX9 | GCCAATTTCTGGCCAAAGCA | CGAGGCTCAATGGGGAGTTT |
β-actin | CAGGCACCAGGGCGTGAT | TAGCAACGTACATGGCTGGG |
MCM, minichromosome maintenance complex component; CHK1, checkpoint kinase 1; DHX9, DEAH (Asp-Glu-Ala-His) box helicase 9.