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. 2020 Sep 30;20(6):311. doi: 10.3892/ol.2020.12174

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — PD induces apoptosis in GBC cells. (A) Flow cytometry was used to analyze PD-treated GBC cells. FITC single-positive scatters in the lower right quadrant are early apoptotic cells, FITC and PI double-positive scatters in the upper right quadrant are late apoptotic (including necrotic) cells. (B) The percentage of apoptotic cells expressed as the mean ± SD. (C) Nuclear morphology was observed using fluorescence microscopy after staining with Hoechst 33342 (magnification, ×200). The experiments were performed three times separately. *P<0.05, **P<0.01 vs. control. PD, Platycodin D; GBC, gallbladder cancer.