Figure 1.

Tumorigenic and metastatic potential of colorectal cancer cells. (A) Soft agar assay was performed to measure the tumorigenic potential of cells. The colonies were imaged and counted after 3 weeks, and representative images of one of the three experiments are shown. (B) Total number of colonies were counted and represented as relative colony units. (C) Cell migration was analyzed using Transwell assay (triplicate/line), and cells that migrated to the lower surface were stained and imaged. Scale bar, 50 µm. (D) Number of cells migrated and stained was counted and represented relative migration units. ***P<0.001 vs. HT-29 cells.