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. 2020 Sep 30;20(6):313. doi: 10.3892/ol.2020.12176

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Copyright: © Rai et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Mitochondrial C-I assembly and activity analysis. (A) BN-PAGE was performed in the isolated mitochondrial preparation of cultured cells. (B) C-I activity was measured using in gel C-I activity assay. Relative band intensities of C-I on (C) BN-PAGE and (D) in-gel assay were quantitated using ImageJ and normalized with total mitochondrial protein loaded (80 µg). (E) Equal loading of mitochondrial proteins is shown by immunoblotting results of an aliquot of the same samples used for BN-PAGE/in-gel assay, with mitochondrial marker protein VDAC. C-, complex; VDAC, Voltage-dependent anion channel; BN-PAGE, Blue native polyacrylamide gel electrophoresis.