Figure S1. Effects of co-culture with the fungus on the bacterial growth.

(A) The spores of A. nidulans were incubated in 200 μl minimal medium for 7 h until they germinated, then precultured B. subtilis were inoculated at a final concentration of OD₆₀₀ = 0.01. Bacterial growth rates measured in 96-well titer plate and shown by OD₆₀₀ every 30 min for 24 h. Error bar: SD, n = 3. F, fungus; B, bacteria. (B) Bacterial genomic DNA was extracted from bacterial monoculture or co-culture with the fungus on the minimal medium agar plates incubated at 30°C for 3 d. Error bar: SD, n = 3. *P < 0.05. (C) The relative expressions of tenA and thiU in the bacterial monoculture or co-culture with the fungus measured by qRT-PCR. The expression of sigA is used as a standard. Error bar: SD, n = 3. **P < 0.01.