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. 2020 Oct 19;21:721. doi: 10.1186/s12864-020-07118-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Principal Component Analysis (PCA) depicted tissue-dependent expression patterns. Circos plot (a) visualization of transcript fold changes and their significance levels in different tissues of alfalfa plants in response to drought stress. (A) Heat map of leaf-derived log2 transcript fold changes, (B) p_values of the DEG in leaf tissues, (C) Heat map of stem-derived log2 transcript fold changes, (D) p_values of the DEG in stem tissues, (E) Heat map of root-derived log2 transcript fold changes, (F) p_values of the DEG in root tissues. b PCA plots are constructed using total exon read counts from ‘.bam’ extension RNA sequenced samples irrespective of genotype and tissue. Transcript level comparisons in ‘a’ were between leaf, stem, and root tissues of drought-stressed SPL13RNAi and EV alfalfa plants. Red and blue colours from the heat map in ‘a’ represent an increased and decreased transcript log2 fold-changes, respectively. P_values are represented with green colours. Circlize (V 0.4.11), an R-software package was used for data visualization [19]. n = 3 biological replicates