Table 1.
Detected candidate proteins interacting with SPL13 by IPMS analysis
| Proteins | Reported roles and description | Sequence coverage (%) | # Peptides | # Spectra | Molecular weight (KDa) |
|---|---|---|---|---|---|
| 50S ribosomal protein L22 | Early stages of 50S ribosomal protein assembly | 18 | 4 | 7 | 21.88 |
| 60s ribosomal protein L11 | Biogenesis of 60S ribosomal subunits | 49.72 | 6 | 11 | 20.67 |
| Arginine biosynthesis bifunctional protein | Involved in the cyclic version of arginine biosynthesis | 26.72 | 7 | 21 | 48.6 |
| Beta-xylosidase/alpha-L-arabinofuranosidase | Releases xylose and arabinose from cell walls | 25.32 | 15 | 30 | 83.67 |
| Cafeic acid 3-O-methlyltransferase (COMT1) | Catalyzes the conversion of caffeic acid to ferulic acid and of 5-hydroxyferulic acid to sinapic acid. | 17.81 | 6 | 31 | 39.92 |
| Cell division control protein 2 homolog 1 | control of the eukaryotic cell cycle | 17.87 | 4 | 11 | 33.46 |
| Chalcone synthase 1 | naringenin-chalcone or chalcone biosynthesis | 15.17 | 5 | 14 | 42.74 |
| Chalcone synthase 2 | 31.62 | 9 | 38 | 42.68 | |
| Chalcone synthase 4 | 28.53 | 8 | 34 | 42.63 | |
| Cyclic beta-(1,2)-glucan synthase | biosynthesis of cyclic beta-(1,2)-glucan | 15.11 | 29 | 86 | 315.55 |
| Dihydroflavonol 4-reductase | anthocyanin biosynthesis | 33.18 | 6 | 12 | 24.37 |
| Eukaryotic translation initiation factor 5A-1 | mRNA-binding protein involved in translation elongation | 32.3 | 3 | 32 | 17.65 |
| Glutamate synthasee [NADH] amyloplastic | assimilation of symbiotically fixed nitrogen into amino acids | 19.51 | 30 | 99 | 240.22 |
| Glutamine synthetase cytosolic isozyme | homeostatic control of glutamine synthesis | 16.85 | 4 | 9 | 39.08 |
| Glutamine synthetase leaf isozyme, chloroplastic | Re-assimilation of the ammonia generated by photorespiration | 14.95 | 6 | 15 | 47.09 |
| Glycogen synthase kinase-3 homolog − 1 | Brassinosteroids signaling and abiotic stress response | 21.41 | 6 | 11 | 46.74 |
| Glycogen synthase kinase-3 homolog − 2 | 25.3 | 7 | 94 | 46.03 | |
| Glycogen synthase kinase-3 homolog − 3 | 37.23 | 11 | 27 | 46.24 | |
| Isoflavone reductase, IFR | Reduces achiral isoflavones to chiral isoflavanones | 13.52 | 4 | 5 | 35.43 |
| Long-distance movement protein | facilitate the cell-to-cell movement | 17.93 | 3 | 3 | 26.67 |
| Malate dehydrogenase, chloroplastic | Catalyzes a reversible NAD-dependent dehydrogenase reaction involved in central metabolism | 16.87 | 3 | 3 | 35.52 |
| Methylthioribose-1-phosphate isomerase | Catalyzes methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P) interconversion | 38.14 | 11 | 15 | 42.24 |
| Mitogen-activated protein kinase homolog | mitogenic induction of symbiotic root nodules | 21.19 | 6 | 32 | 44.37 |
| Nodulation protein D1 | Regulates other nodulation proteins | 15.2 | 3 | 3 | 34.86 |
| Nodulation protein E | synthesize NOD factor fatty acyl chain | 26.68 | 7 | 9 | 41.64 |
| Nodulin-25 | development and function of nodules | 19.51 | 3 | 6 | 27.51 |
| Phenylalanine ammonia-lyase | catalyzes the deamination of phenylalanine to cinnamate and ammonia | 21.38 | 12 | 21 | 78.82 |
| Phosphoenolpyruvate carboxylase | catalyzes the addition of bicarbonate to phosphoenolpyruvate | 17.08 | 11 | 16 | 110.82 |
| Polygalacturonase | depolymerization of pectates | 15.44 | 6 | 29 | 43.93 |
| Replication protein 1A | stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. | 33.13 | 25 | 170 | 111.95 |
| Ribulose bisphosphate carboxylase large chain | Catalyzes the carboxylation of D-ribulose 1,5-bisphosphate and oxidative fragmentation of the pentose substrate | 24.05 | 12 | 21 | 52.59 |
| Ribulose bisphosphate carboxylase small chain | Catalyzes the carboxylation of D-ribulose 1,5-bisphosphate and oxidative fragmentation of the pentose substrate | 46.67 | 7 | 34 | 20.24 |
| RNA-directed RNA polymerase | catalyses synthesis of the RNA strand complementary to a given RNA template | 15.14 | 9 | 34 | 93.6 |
| RNA-directed RNA polymerase 2a | required for cytoplasmic and chromatin RNA silencing pathways | 16.91 | 9 | 13 | 93.46 |
| Serine/threonin-protein phosphatase PP1 | essential for cell division and glycogen metabolism by dephosphorylating substrates | 29.6 | 8 | 97 | 36.23 |
| Serine/threonin-protein phosphatase PP2A | Dephosphorylates and activates the actin-depolymerizing factor ADF1 | 18.85 | 3 | 5 | 35.69 |
| Sucrose synthase | Sucrose-cleaving enzyme that provides UDP-glucose and fructose | 15.65 | 8 | 31 | 92.27 |
| Tyrosine recombinase XerC | Catalyze the cutting and rejoining of the recombining DNA molecules | 15.72 | 3 | 3 | 33.75 |
| Vacuolar membrane protease | vacuolar sorting and osmoregulation | 21.96 | 14 | 45 | 112.35 |
| Vacuolar protein sorting/targeting protein 10 | sorting receptor in the Golgi compartment required for the intracellular sorting and delivery of soluble vacuolar proteins | 18.45 | 22 | 67 | 161.33 |
Candidate proteins were considered when at least three peptides with six spectra were present from the protein while undergoing the database search as specified in the method part of the article. Thermo Q-Exactive Orbitrap mass spectrometer coupled to an Easy-nLC 1000 nano LC system were used to analyze samples