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. 2020 Oct 19;51:133. doi: 10.1186/s13567-020-00857-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Construction and expression of recombinant gp90 of REV. A The ORF of recombinant gp90 contained the whole length gp90 (1083 bp), the signaling peptide (87 bp) at the N-terminus, and a his-tag at the C-terminus. B Western blot identification of recombinant gp90 with anti-gp90 mAb at different expression time points. C The gray value assay for expressed gp90 at variable time points.