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. 2019 Dec 4;15(6-7):618–631. doi: 10.1080/15592294.2019.1700004

Figure 1.

Figure 1.

Reactivation of methylated P16 alleles in H1299 cells 6 days after P16-TET transient transfection. (a) Core sequences of the catalytic domain (CD) of the wildtype TET1 gene and its T > C H1671Y-mutant counterpart. The P16-TET pcDNA3.1 and pTRIPZ ‘Tet-on’ expression vectors are also illustrated and used in transient and stable transfection studies, respectively. (b) The results of qRT-PCR to detect the P16 mRNA levels in H1299 cells transiently transfected with the pcDNA3.1 empty control, P16-TET and its H1671Y mutant counterpart vectors. (c) Immunofluorescence staining with the mouse monoclonal antibody against the human P16 protein (Ventana Roche-E6H4, USA). These experiments were performed in triplicate and repeated at least one time. Bar: 50 μm.