Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 4;15(6-7):618–631. doi: 10.1080/15592294.2019.1700004

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Informa UK Limited, trading as Taylor & Francis Group

PMC Copyright notice

Figure 3. — P16-TET induces hydroxymethylation of P16 CpG islands and reactivates expression of methylated-P16 alleles in AGS cells. (a) Bisulphite-DHPLC analysis for detecting methylated-P16 (P16M) and unmethylated-P16 (P16U) PCR products for the exon-1 antisense strand in P16-TET-transfected AGS cells with different doxycycline induction times. (b) The TAB-DHPLC analysis detected the hydroxymethylated P16 (P16H) PCR products and nonhydroxymethylated P16 (P16N) PCR products. (c and d) Bisulphite and TAB sequencing for detecting 5mC and 5hmC sites, respectively, in the same PCR products as were analysed by DHPLC. (e and f) The results of RT-PCR and Western blot analysis for detecting P16 reactivation in AGS cells.