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. 2019 Dec 4;15(6-7):618–631. doi: 10.1080/15592294.2019.1700004

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Figure 5. — Characterization of P16H in FACS-sorted subpopulations of AGS cells with various levels of P16 expression reactivation. (a) FACS-sorting of P16-TET stably transfected AGS cells with and without DAC treatment. The confocal images of the P16 protein staining status are also attached. Bar: 50 μm. The sorted AGS cells not treated with doxycycline only expressed P16-TET at the baseline level. (b) Detection of the DNA hydroxymethylation status of P16 alleles in various FACS sorted AGS subpopulations with strong, weak, and no P16-immunostaining (P16(+)/(±)/(-)) in the TAB-MSP analysis. (c) The results of TAB-DHPLC for the P16 CpG islands in three subpopulations. (d) The results of TAB sequencing for the antisense strand of P16 exon-1 in P16(+) and P16(-) subpopulations.