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. 2020 Oct 6;11:549531. doi: 10.3389/fmicb.2020.549531

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Copyright © 2020 Wambui, Eshwar, Aalto-Araneda, Pöntinen, Stevens, Njage and Tasara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotypic evaluation of high nisin sensitivity L. monocytogenes field strains with natural vir operon mutations. (A) Bacitracin sensitivity comparison based on bacitracin induced growth inhibition (ΔPAUC) and quantification of VirR dependent dltA (B) and mprF (C) mRNAs in high nisin sensitivity vir operon mutant strains (N12-1859, N12-0160, N12-2449, and LK132) compared with corresponding clonal control strains (JHM270, N11-1251, and HT45E) without vir operon mutations. Data presented is based on three independent biological experiments. *P > 0.05 significant differences that were identified using one-way ANOVA and Tukey post hoc (groups with more than two strains) pairwise comparison of the strains.