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. 2020 Jul 15;10(7):200118. doi: 10.1098/rsob.200118

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© 2020 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 5. — Representative Ca²⁺ traces resulting from egg activation by various methods. Top row, chemical inducers of artificial egg activation (AEA) in bovine eggs [234]. Bottom row, in vitro fertilization (IVF) [68], Plcz1 cRNA injection [123] and intracytoplasmic sperm injection (ICSI) [235] in mouse eggs; ICSI using sperm lacking PLCζ activity, in medium containing CaCl₂, followed by two treatments with ionomycin in human egg [235]. Scale bar applies to all traces. Traces adapted from indicated references.