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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Nat Methods. 2020 Sep 14;17(10):1018–1024. doi: 10.1038/s41592-020-0929-2

Extended Data Figure 9: Collage of examples showing sf-tPAINT of human platelets treated with cytoskeletal inhibitors.

Extended Data Figure 9:

(a) Time-resolved 8.5 pN tPAINT of platelets pre-treated with 50 μM CK666 for 30 min before being seeded on sf-tPAINT probes. (b) Time-resolved 8.5 pN tPAINT image of platelet before and after treatment with 10μM ML-7 (MLCK inhibitor). (c). Representative examples of images for cells treated with inhibitors displayed with a 5x density filter and kinetic filter. Human platelets treated with vehicle (DMSO) as control (n = 3 independent experiments), human platelets treated with CK666 (50μM) after being seeded on the surface (n = 2 independent experiments), human platelets pretreated with CK666 (50 μM) for 30 min before being seeded on the surface (n = 3 independent experiments), human platelets treated with ML-7 (MLCK inhibitor, 10 μM) after being seeded on the surface (n = 3 independent experiments). Three examples are shown for each condition.