Figure 13.

Para enrichment at the Distal Axonal Segment (DAS) is seen in the mushroom body (MB) neurons of the adult central nervous system (CNS). A, B, 201Y-GAL4 is expressed specifically in the neurons of the MB. C,C′,C′′,C′′′, Expression of mCD8:GFP in the MB clearly marks the cell body (CB), axon (Ax), and the Calyx where the neurons receive dendritic input. Para-mCherry is enriched at the DAS in the axon of the MB neurons, distal to the calyx. D,D′,D′′, UAS-DenMark expression in the MB neurons using 201Y-GAL4 colocalized with UAS-mCD8::GFP in the cell body and calyx region of the neurons but not in the peduncle beyond the DAS.