Table 2.
para-positive cluster 3 of the single-cell transcriptomic atlas is enriched for genes required for neurotransmission and ion transporta
| GO molecular function complete | D. melanogaster - REFLIST (13781) | Cluster 3 (96) | Cluster 3 (expected) | Cluster 3 (over/under) | Cluster 3 (fold enrichment) | Cluster 3 (p) |
|---|---|---|---|---|---|---|
| Syntaxin binding (GO:0019905) | 26 | 5 | 0.18 | + | 27.61 | 2.87E-03 |
| Ion transmembrane transporter activity, phosphorylative mechanism (GO:0015662) | 21 | 4 | 0.15 | + | 27.34 | 3.32E-02 |
| Hormone activity (GO:0005179) | 49 | 8 | 0.34 | + | 23.44 | 6.67E-06 |
| Neuropeptide hormone activity (GO:0005184) | 33 | 5 | 0.23 | + | 21.75 | 8.16E-03 |
| SNARE binding (GO:0000149) | 48 | 6 | 0.33 | + | 17.94 | 2.53E-03 |
| ATPase-coupled cation transmembrane transporter activity (GO:0019829) | 50 | 6 | 0.35 | + | 17.23 | 3.14E-03 |
| ATPase-coupled ion transmembrane transporter activity (GO:0042625) | 54 | 6 | 0.38 | + | 15.95 | 4.74E-03 |
| Receptor ligand activity (GO:0048018) | 97 | 10 | 0.68 | + | 14.8 | 4.30E-06 |
| Neurotransmitter receptor activity (GO:0030594) | 70 | 7 | 0.49 | + | 14.36 | 1.35E-03 |
| Signaling receptor activator activity (GO:0030546) | 101 | 10 | 0.7 | + | 14.21 | 6.16E-06 |
| Receptor regulator activity (GO:0030545) | 104 | 10 | 0.72 | + | 13.8 | 8.00E-06 |
| Primary active transmembrane transporter activity (GO:0015399) | 121 | 7 | 0.84 | + | 8.3 | 3.97E-02 |
| Signaling receptor binding (GO:0005102) | 257 | 12 | 1.79 | + | 6.7 | 4.43E-04 |
| Monovalent inorganic cation transmembrane transporter activity (GO:0015077) | 240 | 10 | 1.67 | + | 5.98 | 1.19E-02 |
| Inorganic cation transmembrane transporter activity (GO:0022890) | 330 | 13 | 2.3 | + | 5.66 | 8.90E-04 |
| Cation transmembrane transporter activity (GO:0008324) | 346 | 13 | 2.41 | + | 5.39 | 1.49E-03 |
| Transmembrane signaling receptor activity (GO:0004888) | 342 | 11 | 2.38 | + | 4.62 | 4.31E-02 |
| Signaling receptor activity (GO:0038023) | 401 | 12 | 2.79 | + | 4.3 | 3.67E-02 |
| Molecular transducer activity (GO:0060089) | 402 | 12 | 2.8 | + | 4.29 | 3.76E-02 |
| Inorganic molecular entity transmembrane transporter activity (GO:0015318) | 511 | 15 | 3.56 | + | 4.21 | 4.13E-03 |
| Ion transmembrane transporter activity (GO:0015075) | 535 | 15 | 3.73 | + | 4.02 | 7.10E-03 |
| Molecular function regulator (GO:0098772) | 568 | 15 | 3.96 | + | 3.79 | 1.43E-02 |
| Transmembrane transporter activity (GO:0022857) | 731 | 17 | 5.09 | + | 3.34 | 1.76E-02 |
| Transporter activity (GO:0005215) | 791 | 17 | 5.51 | + | 3.09 | 4.75E-02 |
| Unclassified (UNCLASSIFIED) | 2872 | 10 | 20.01 | – | 0.5 | 0.00E + 00 |
aPANTHER overenrichment to determine enriched GO molecular functions in the top 100 enriched genes in cluster 3 of the single-cell third instar larval brain transcriptomic atlas shows that cluster 3 is enriched for genes involved in neuronal transmission, ATP synthesis, and ion transport. Process upregulated in actively firing neurons.