Figure 2.

Expression levels of CM in different transgenic lines. A, Western blot showing the levels of arrestin and rhodopsin in whole retina in WT mice (C57BL), and different transgenic lines, with indicated amounts of corresponding purified proteins used as standards. Arr1 and rhodopsin were measured by quantitative Western blot, as described previously (Song et al., 2011; for details, see Materials and Methods). Blots of representative samples (of three per genotype used) are shown. GAPDH was used as a loading control. B, Quantification of the expression of Arr1 and rhodopsin in whole mouse retina with indicated genotypes. Mean ± SD values are shown. Statistical significance of the differences (compared with WT) is shown, as follows: **p < 0.01; ***p < 0.001.