Table 2.
Antibodies used in the current study
| Antibody name [cell type] | Immunogen | Source, catalog number, RRID | Antibody type | Dilution |
|---|---|---|---|---|
| Calbindin (CaBP) [DB3a] | CaBP-D-28 kDa purified from chicken gut; hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse | Swant, 300, lot: 07(F); RRID: AB_10000347 | Mouse monoclonal | 1:10,000 |
| Calbindin (CaBP) [DB3a] | Recombinant rat calbindin D-28k | Swant, CB38, lot: 5.5 RRID: AB_10000340 | Rabbit polyclonal | 1:20,000 |
| CD15 [DB3b] | U-937 histiocytic cell line, purified from tissue culture supernatant or ascites by affinity chromatography | BD Biosciences, 559045; RRID: AB_397181 | Mouse monoclonal | 1:100 |
| Cone arrestin (7G6) [cones] | Developed using macaque light-adapted retinal extract as immunogen; gives single band of 44–46 kDa on Western blottings of human retina | Gift from Prof. Peter Macleish, Morehouse School of Medicine, Atlanta, GA; RRID: AB_2314215 | Mouse monoclonal | 1:500 |
| Parvalbumin [ganglion cells] | Purified from carp muscles; stains the 35Ca-binding spot of parvalbumin | Swant, 235, lot: 10–11 (F); RRID: AB_10000343. | Mouse monoclonal | 1:20,000 |
| RNA-RBPMS [ganglion cells] | Synthetic peptide corresponding to amino acid residues from the N-terminal region of the rat RBPMS sequence, recognizes the 24-kDa RBPMS protein | Lubio, PhosphoSolutions, 1832-RBPMS, lot: NB916o; RRID: AB_2492226 | Guinea pig polyclonal | 1:500 |
| RNA-RBPMS [ganglion cells] | Recombinant protein encompassing a sequence within the left region of human RBPMS | Gene Tex, Sapphire Bioscience, GTX118619, lot: 40415 RRID: AB_10720427 | Rabbit polyclonal | 1:500 |
| Recoverin [OFF midget bipolar cells] | Recombinant human recoverin, recognizes single band of 26 kDa | Millipore, AB5585, lot: LV1480447 RRID: AB_2253622 | Rabbit polyclonal | 1:10,000 |
| S-Opsin [S-cones] | Peptide corresponding to amino acids 1 −50 from the N terminus of the opsin protein encoded by OPN1SW of human origin | Santa Cruz, sc-14363, lot: A2813; RRID: AB_2158332 | Goat polyclonal | 1:100 |