Fig. 2.

Detection of IS/1494/06 in kidney tissue samples and determination of the viral load by real-time RT-PCR of a conserved sequence of the IBV genome. The control group remained uninfected up to the end of the experiment, and the infection of challenge group was confirmed by real-time RT-PCR. , 2 dpi control group; , 2 dpi infected group; , 7 dpi control group; , 7 dpi infected group; , negative RT-PCR standard; , standard 10⁴; , standard 10⁵; , standard 10⁶; , standard 10⁷; , standard 10⁸; , positive RT-PCR standard