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. 2020 Oct 6;10:573318. doi: 10.3389/fonc.2020.573318

FIGURE 3.

FIGURE 3

PHF20 regulates the expression of WISP1 by binding to its promoter. (A) BT115 and U87 cell extracts were immunoprecipitated with anti-PHF20 antibody, followed by immunoblotting with anti-WDR5 antibody. (B) Analysis of PHF20 binding to the promoter regions of WISP1 in glioma cells by ChIP–qPCR assay with PHF20-specific antibody. The data are presented as fold enrichment relative to input DNA. ChIP-qPCR primers are listed in Supplementary Table 2. (C) Analysis of WDR5 binding to the promoter regions of WISP1 in glioma cells by ChIP–qPCR assay with WDR5-specific antibody. The data are presented as fold enrichment relative to input DNA. (D) ChIP-qPCR analysis of WDR5 binding to the promoter region of WISP1 in WDR5 knockdown cells. The rate of PHF20 binding to the WISP1 promoters decreased in WDR5 KD BT115 and U87 cells compared to the control cells. (E) Western blot analysis of PHF20 and WISP1 expression in PHF20 KO, PHF20 Teton, and relative control cells. (F) IHC staining of WISP1 in xenografts. Scale bar, 50 μm.