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. 2020 Oct 6;10:573318. doi: 10.3389/fonc.2020.573318

FIGURE 4.

FIGURE 4

PHF20 indirectly regulates BGN expression through WISP1. (A) Protein interaction analysis (PPI) of WISP1. (B) The expression level of BGN was analyzed by qPCR in WISP1 KD and control glioma cells. Cells transfected with WISP1 shRNA or control shRNA were regarded as WISP1 KD cell or control cell, respectively. (C) Western blot analysis of BGN in WISP1 KD and control glioma cells. (D) The expression level of WISP1 was analyzed by qPCR in BGN KD and control glioma cells. Cells transfected with BGN shRNA or control shRNA were regarded as BGN KD cell or control cell, respectively. (E) Western blot analysis of WISP1 in BGN KD and control glioma cells. (F) The expression levels of WISP1 and BGN were analyzed by qPCR in WDR5 KD and control glioma cells. Cells transfected with WDR5 shRNA or control shRNA were regarded as WDR5 KD cell or control cell, respectively. (G) Western blot analysis of WISP1 and BGN in WDR5 KD and control glioma cells. (H) Control GBM cells (control shRNA), WISP1 KD (WISP1 shRNA), BGN KD (BGN shRNA), WISP1 + BGN KD (WISP1 shRNA + BGN shRNA), and PHF20 KO cells (PHF20 sgRNA) were plated in a 96-well plate using 200 μl medium. Cell viability was assayed using MTT assay (540 nm). All the groups in BT115 and U87 cells showed significantly reduced cell viability compared to the control cells. Scale bar, 50 μm. Data are plotted as mean ± SD of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 compared with controls using Student’s t-test.