The proliferation of ALL cells was monitored by counting viable cells daily (top panel), and the cell-cycle distribution was determined by propidium iodide staining and flow cytometry (middle panel). Compared to non-target control cells, ARID5B knockdown cells had higher percentages of cells in the G0/G1 phases but lower percentages in the S and G2/M phases. At the molecular level, ARID5B knockdown led to consistent upregulation of p53 and p21 and downregulation of phosphorylated Rb in ALL cell lines (bottom panel). Experiments were performed in Nalm6, SEM, and UOC-B1 cells (A, B, and C, respectively).