Figure 5.

Confocal live images of HeLa incubated for 24 h, after 15-min illumination. (A) Untreated control, (B) treated with CNOs, (C) treated with 3/CNOs at a mass concentration of 10 μg mL⁻¹, (D) treated with 3, at the corresponding concentration of 2.3 × 10⁻⁷ M. Nuclei were stained with Hoechst 33342 (blue), whereas green refers to the I₂-BODIPY signal. Scale bars (A–D) = 50 μm. The presence of 3 or 3/CNO is indicated by the green fluorescence signal, while intracellular oxidative damage is visible as red color (or yellow when overlapping with green). Scale bar = 50 μm.