Skip to main content
. Author manuscript; available in PMC: 2021 Sep 30.
Published in final edited form as: Biochem J. 2020 Sep 30;477(18):3499–3525. doi: 10.1042/BCJ20200065

Figure 4. Molecular mechanism of loading the second Cdt1-bound Mcm2–7 hexamer onto origin DNA.

Figure 4.

After the formation of OCCM, ORC and Cdc6 dissociate from the C-tier AAA+ side of the loaded Mcm2–7. ORC binds to B2 element of the origin DNA and the N-tier side of the first Mcm2–7, forming the MO complex. Cdc6 next binds to MO to recruit the second Cdt1-bound Mcm2–7, leading to the eventual assembly of the Mcm2–7 double hexamer.