Fig. 2.

mRNA expression level of TNF-α, IL-18, IL-6, and IL-1β in the activated PBMCs isolated from Nano-curcumin treated and placebo group. The expression level of IL-1β and IL-6 decreased after treatment with Nano curcumin compared with the pre-treatment state (P = 0.0017 and 0.0003, respectively) and compared with the placebo group (P = 0.0001 for both). There were no statistically significant differences in the expression level of IL-18 and TNF-α before and after intervention in Nano-curcumin group, and in Nano-curcumin group compared with placebo groups (Nano-curcumin group number = 20, placebo group number = 20). Evaluation of the mRNA expression level was done using Real-time PCR technique. Paired T test was used for statistical analysis and P < 0.05 was considered statistically significant.