FIGURE 4.

Sch affects podosome belt formation and inhibits osteoclast resorptive function. A, Representative images of podosome belt formation in OCs treated with different concentrations of Sch. Images were observed by confocal microscopy. Scale bar = 200 μm. OC, osteoclast. B, Number of nuclei per osteoclast and average OC area was quantified. C, Hydroxyapatite‐coated plates were used to detect osteoclast resorptive activity at different concentrations of Sch. Scale bar = 500 μm. D, Quantification of pit area and resorbed area. (n = 3 per group). E, Bone resorption‐related proteins CTSK, MMP9 and V‐ATPase D2 are detected by WB. An inhibition of bone resorptive effects was found in different concentrations of Sch. The data are shown as means ± SD. ns, no significance. *P < .05, **P < .01