Rho1 regulates the function of the stem cell niche in Drosophila testes. A, Apical tips of control, nos > Rho1 RNAi and tj > Rho1 RNAi testes labelled with Vasa (red) and 1B1 (green). Red arrowheads indicate representative punctate fusomes, and white arrowheads indicate representative branched fusomes. B, The number of punctate fusomes in the control, nos > Rho1 RNAi and tj > Rho1 RNAi testes. C, The number of branched fusomes in the control, nos > Rho1 RNAi and tj > Rho1 RNAi testes. D, Apical tips of control, nos > Rho1 RNAi and tj > Rho1 testes labelled with Zfh1 (red) and Eya (green). Yellow arrows indicate representative Zfh1&Eya‐positive cells. E, The number of Zfh1‐positive cells in the control, nos > Rho1 RNAi and tj > Rho1 RNAi testes. F, The number of Eya‐positive cells in the control, nos > Rho1 RNAi and tj > Rho1 RNAi testes. G, The number of Zfh1&Eya‐positive cells in the control, nos > Rho1 RNAi and tj > Rho1 RNAi testes. DNA was stained with Hoechst 33 342 (blue). **P < .01, ***P < .001. Scale bars, 20 µm