Figure 6.

The mechanism of miR‐130a‐3p promoting osteogenic differentiation of ADSCs through Wnt signalling pathway. A‐B, The expression of osteogenic‐related protein and key protein of Wnt signalling pathway analysed by Western blot on 3rd and 7th day. C‐D, The expression of ALP and RUNX2 mRNA detected by qPCR. E‐F, ALP activity detected by ALP assay kit. G, The diagram of the mechanism that exosomal miR‐130a‐3p regulates osteogenic differentiation of ADSCs by mediating Wnt signalling pathway. Notes:*represents significant differences between control and other groups without DKK1 treatment; *represents significant differences between no treatment and DKK1 treatment group; and #represents significant differences between lenti‐control and overexpression groups, or lenti‐control and knockdown groups without DKK1 treatment. *P < .05; *P < .05; #P < .05