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. 2020 Sep 14;53(10):e12903. doi: 10.1111/cpr.12903

Figure 4.

Figure 4

Transcriptional repression of p21 by EIF1AX. A, Diagram of human p21 promoter sequence. P1−5 show the regions of the p21 promoter detected by the paired primers. B, EIF1AX was recruited to p21 promoter. Soluble chromatin in MCF‐7 cells was immunoprecipitated with anti‐EIF1AX, rabbit normal IgG antibody served as control. qRT‐PCR analysis of the final extracted DNA using the primers specific to the proximal promoter region of the p21 gene. C, The effect of EIF1AX on p21 promoter activity. Luciferase assay of MCF‐7 cells transfected with p21‐Luc reporter construct and 0, 0.1, 0.2 and 0.4 µg/well EIF1AX expression construct. D, The effect of EIF1AX and p21 knock‐down on cell proliferation. Cell cycle analysis of control, EIF1AX knock‐down and EIF1AX‐ and p21‐knock‐down MCF‐7 cells by flow cytometry. The data represent the results from three independent experiments. E, Western blotting analysis of p21 in EIF1AX‐ and p21‐knock‐down MCF‐7 cells. F, Quantification of proteins in (E)