Figure 5.

BMI1 and H2Aub Genomic Distribution and Changes in Its Enrichment at Specific Genomic Regions with Chemical Inhibition of BMI1

(A and B) ChIP-seq heatmap analysis of BMI1-enriched genomic loci (A) and KEGG pathway enrichment for BMI1 peaks (B) in SU-DIPG04 and SF8628 cells.

(C) ChIP-seq heatmaps of BMI1 and H2Aub in SF8628 cells treated with DMSO (blue) or PTC028 (orange).

(D and E) Average profiles of BMI1 peaks (D) and H2Aub (E) in SU-DIPG04 cells treated with DMSO (green) or PTC028 (orange).

(F) KEGG pathway changes in BMI1 peak enrichment in SU-DIPG04 cells after treatments.

(G) Genome browser views representing the results of ChIP-seq for BMI1 and H2Aub and the results of RNA-seq for the genes GLUL, PTGDS, and SFRP2 in SU-DIPG04 cells.

All ChIP-seq peaks are relative to TSS (±3 kb). Asterisks indicate pathways of differentiation. See also Figure S6.