Figure 6.

ADRA2A blocked PI3K/AKT/mTOR pathway in HeLa and SiHa cells. (A) GSEA suggested that ADRA2A expression was correlated with PI3K/AKT/mTOR pathway. (B) The expressions of p-PI3K, PI3K, p-AKT, AKT, p-mTOR and mTOR in HeLa cells were measured by Western blot. (C) The expressions of p-PI3K, PI3K, p-AKT, AKT, p-mTOR and mTOR in C-33A cells were measured by Western blot. (D) After treatment of LY294002, the expressions of p-PI3K, PI3K, p-AKT, AKT, p-mTOR and mTOR in HeLa and C-33A cells were measured by Western blot. **P < 0.01, vs pcDNA3.1-NC group; ^##P < 0.01, vs si-NC group (B and C); **P < 0.01, vs pcDNA-ADRA2A group, ^##P < 0.01, vs si-ADRA2A group (D).