Table 2. Summary of LAMP-sequencing results.
The cross tabulation of RT-qPCR and RT-LAMP assay results shown in Table 1 have been split into samples where sequencing of RT-LAMP reaction products (LAMP-sequencing) was positive (Pos), negative (Neg), or inconclusive (too few reads) (see also Fig. 4).
RT-LAMP | |||||||
CT | Pos | Neg | Sum | ||||
LAMP- sequencing |
Pos | RT-qPCR | Pos | 0–25 | 49 | 0 | 49 |
25–30 | 28 | 0 | 28 | ||||
30–35 | 4 | 0 | 4 | ||||
35–40 | 0 | 0 | 0 | ||||
Neg | Neg | 0 | 0 | 0 | |||
Neg | RT-qPCR | Pos | 0–25 | 0 | 0 | 0 | |
25–30 | 0 | 2 | 2 | ||||
30–35 | 0 | 16 | 16 | ||||
35–40 | 0 | 16 | 16 | ||||
Neg | Neg | 2 | 637 | 639 | |||
Too few reads | RT-qPCR | Pos | 0–25 | 2 | 0 | 2 | |
25–30 | 0 | 0 | 0 | ||||
30–35 | 0 | 0 | 0 | ||||
35–40 | 0 | 0 | 0 | ||||
Neg | Neg | 0 | 12 | 12 | |||
Sum | 85 | 683 | 768 |