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. 2020 Oct 8;16(10):e1008987. doi: 10.1371/journal.ppat.1008987

Fig 5. M2-2 overexpression early in infection inhibits RSV infection.

Fig 5

A) Vero cells were transfected with 200 ng of M2-2-Myc mRNA 24 h pre-infection, 4 hpi, or 10 hpi. Cells were infected with RSV at a MOI of 3 and fixed 24 hpi. Cells were stained for M2-2 via Myc (green), and panRSV (red). Representative single plane images are shown, with a scale bar of 10 μm. Control images are presented in S8 Fig. B) Vero cells were infected with RSV at a MOI of 3 and transfected with 200 ng of M2-2-Myc or M2-1-HA mRNA 3 hpi. Cells were fixed at 24 hpi and stained for M2-2 via Myc (green) or M2-1 via HA (green), and panRSV (red). Representative extended focus images are shown, with a scale bar of 10 μm.