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| Volume per reaction (μl) | |
|---|---|
| RNase-free Water | 11.1 |
| 2019-nCoV MOM (primer and probe mix) | 6 |
| 5X Real-time One-step Buffer | 6 |
| Real-time One-step Enzyme | 2.4 |
| Internal control (RP-IC) | 1.5 |
| Sample after centrifugation and heating | 3 |
| Total volume | 30 |
