Fig 2. Validation of macrophage phenotype in vitro.

Peritoneal macrophages (PM) from both male and female WT, M2^-/-, and M2-OE mice that had been treated with zymosan, were polarized into M1 and M2 phenotypes as described in Materials and Methods. Cells were then harvested; total RNA was isolated, and TaqMan RT-PCR was performed using ARG-1 (for M2 phenotype)- and NOS2 (for M1 phenotype)- specific primers. Expression of ARG1 (A) and NOS2 (B) mRNA was normalized to that of GAPDH RNA. Each bar represents the mean ± SEM from two independent experiments (N = 4).