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. 2020 Oct 8;16(10):e1008971. doi: 10.1371/journal.ppat.1008971

Fig 3. Phagocytosis assay on in vitro derived bone marrow-derived (BM) macrophages.

Fig 3

Cells from BM-derived WT, M2-/-, and M2-OE mice were cultured, differentiated into macrophages, and cultured in 24-well plates as described in Materials and Methods. After overnight culture, adhered cells were infected with HSV-1 and incubated with latex beads-rabbit IgG-FITC complex. Cells were stained with F4/80 AF 564 antibody and subjected to flow cytometry analysis. Panel A) representative plots of WT, M2-/-, and M2-OE macrophages; and B) Percentage phagocytosis plots from three separate experiments.