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. 2020 Oct 8;16(10):e1008971. doi: 10.1371/journal.ppat.1008971

Fig 4. Effect of macrophage polarization on HSV-1 replication in vitro.

Fig 4

WT, M2-/-, and M2-OE mice were treated with zymosan and PM from WT, M2-/-, and M2-OE mice were harvested. Isolated cells were mock treated for 24 hr (A), incubated for 24 hr with either IL-4 to generate M2 macrophages (B) or IFN-γ and LPS to generate M1 macrophages (C). Cells were then infected with 10 PFU/cell of McKrae virus for 1 hr. Infected cells were harvested at 12 and 24 hr PI and virus titers were determined using a standard plaque assay on RS cells. Each point represents the mean ± SEM from two independent experiments (N = 4).