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. 2020 Oct 20;16(10):e1008945. doi: 10.1371/journal.ppat.1008945

Fig 4. HBV RNA-virion utilizes MVB pathway for egress.

Fig 4

Huh7 cells in 6-well plate were cotransfected with the pCMVHBV (wt HBV) or pCMVHBV-Y63D (Y63D) and control vector, or GFP/DsRed-tagged wildtype (wt) VPS4A or dominant-negative (DN) version of VPS4A at a 9:1 DNA weight ratio (2.7:0.3 μg). Cells were harvested at day 5 post-transfection and the intracellular total viral RNA, encapsidated pgRNA, HBc and VPS4A proteins were analyzed by Northern and Western blot, respectively. HBV particles in culture fluids were analyzed by particle gel assay. HBV DNA/RNA in virions and naked capsids were detected by (-) strand- and (+) strand-specific probes.